ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (12): 1964-1970.doi: 10.11843/j.issn.0366-6964.2014.12.008

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Detection of EphA1 Expression and Methylation during Embryo Implantation in Meishan Pigs

FU Yan-feng1,LI Lan2,3,ZHOU Yan-hong1,4,LI Bi-xia1,ZHAO Wei-min1,ZHAO Fang1,REN Shou-wen1*   

  1. (1.The Key Laboratory of Animal Breed Improvement and Reproduction, Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;3.National Research Center of Veterinary Biologicals Engineering and Technology,Nanjing 210014,China;4.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
  • Received:2014-07-28 Online:2014-12-23 Published:2014-12-23

Abstract:

Erythropoietin-producing hepatocellular receptor and ligand A1 (EphA1) plays a role in cell migration and adhesion during embryonic development in various mammals.The purpose of the study is to analyze EphA1 methylation effect on its expression during embryo implantation.In this study,the mRNA expression,protein expression and CpG island methylation of EphA1 was investigated in Meishan pigs using real-time quantitative PCR,Western blot and bisulfate sequencing technology.The results showed that EphA1 mRNA expression increased from 13 to 18 d and decreased from 18 to 24 d (P<0.05) in endometrium implantation sites of Meishan pigs,while EphA1 protein expression decreased from 13 to 24 d,and expression in pregnant sows was significantly higher than that of non-pregnant sows (P<0.05) during pre-implantation (13 d),mid-implantation (18 d) and post-implantation (24 d) period.About CpG island methylation level of EphA1,it had a highest methylation pre-implantation period,followed by mid-implantation and post-implantation,this indicated EphA1 CpG island methylation level was consistent with protein expression level during pre-implantation,mid-implantation and post-implantation period.These findings suggest that CpG island methylation of EphA1 might affect its expression and further affect the regulation of swine embryo implantation.

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